SOX2, c-MYC and ABCG2 are upregulated transcriptionally by the Wnt/β-catenin signaling pathway,38, 39, 40 which is aberrantly activated in HCC and has important roles in CSC maintenance.41, 42, 43 Interestingly, we found that ZNF687 overexpression significantly increased the luciferase activity of the TOPflash/FOPflash reporter but that ZNF687 downregulation decreased it, suggesting that ZNF687 may be involved in the activation of Wnt/β-catenin signaling (Supplementary Figure 6A). This evidence concerns the gene ZNF687 and hepatocellular carcinoma.