Due to the lack of reliable, cell type independent NOTCH target genes (Ntziachristos et al., 2014), we used EMSA as a surrogate model for the detection of NOTCH/CSL transcription factor complexes and found that human cell lines derived from melanoma (518A2), HCC (Hep3B, HCC-3, SNU398), and pancreas-CA (PANC-1) express high amounts of nuclear NOTCH2. The gene discussed is RBPJ; the disease is melanoma.