NOTCH2 and B-cell chronic lymphocytic leukemia: As shown in Figure 1A lane 4, treatment of 518A2, HEP3B, HCC-3, SNU398, and PANC-1 cells with 5 μM DAPT, a concentration which we previously showed that it inhibits NOTCH2 signaling in GSI sensitive CLL cases (Hubmann et al., 2010, 2013), had no effect on the NOTCH2 transcription factor complex after 1 day of incubation.