Because we observed upregulation of genes involved in glutathione regulation (PRDX2 and GPX3) in BMI1Over myoblasts, we set out to further analyze the GSH/GSSG ratio in DMD cultures, where a reduction in the GSH/GSSG ratio is considered indicative of oxidative stress (Owen and Butterfield, 2010). Here, GPX3 is linked to Duchenne muscular dystrophy.