We transfected human colon cancer HCT116 cells with cDNA coding for full-length MUC1, MUC1 with intra- or extra-cellular domain (VNTR region) depletion (Figure 1a) and generated full-length MUC1 transfectants (HCT116MUC1Full), MUC1 intracellular domain-depleted (HCT116MUC1ΔCT) and MUC1 extracellular tandem repeat domain depleted mutants (HCT116MUC1ΔTR) (Figure 1b). Here, MUC1 is linked to malignant colon neoplasm.