Gross cystic disease fluid protein-15 (GCDFP-15) and vimentin staining are used most frequently in this setting as breast carcinomas are usually positive for GCDFP-15 and negative for vimentin in most cases, whereas primary ovarian carcinoma is usually negative for GCDFP-15 and often variably positive for vimentin [57]. This evidence concerns the gene VIM and ovarian carcinoma.