ACE and osteosarcoma: Using mice and rats of different ages, weight ranges, sexes, strains and various breeding and housing conditions, CIBP studies employ a wide variety of cultured cancer cell lines including lung cancer (e.g., Lewis Lung), prostate cancer (e.g., PC3N, ACE-1, AT-3), breast cancer (e.g., 66.1, 4T1, MDA-MB-231, MDA-MB-231-TXSA, MRMT1), colon cancer (e.g., Colon-26), osteosarcoma (NCTC 2472) and fibrosarcoma (e.g., MC57G) (Pacharinsak and Beitz, 2008; Currie et al., 2013, 2014; Lozano-Ondoua et al., 2013; Falk and Dickenson, 2014; Slosky et al., 2015).