These results confirm that loss of Wnt8b function in the Foxg1−/− null background leads to a specific increase in Pax2 expression in the anterior (nasal) tips of the OF, strongly suggesting that this may contribute to the rescue of the coloboma phenotype in the Foxg1−/−;Wnt8b−/− double mutant. The gene discussed is FOXG1; the disease is coloboma.