Next, to test the involvement of cleaved Caspase-3 in AML-cell death, we pretreated MV4-11 cells and primary AML leukopheresis samples with a Caspase-3 inhibitor, Z-DEVD-FMK for 45 minutes, and treated with increasing concentrations of AHCC for 24 hours. The gene discussed is CASP3; the disease is acute myeloid leukemia.