Optic neuritis began at 2 weeks post immunization (2WPI) and peaked at 3WPI, which is evidenced by the infiltration of inflammatory cells in ON stained with hematoxylin and eosin (H&E) and also recognized by microglial marker Iba1 and T cell marker CD3 (Figure 1b). The gene discussed is AIF1; the disease is optic neuritis.