We had previously confirmed the intestinal I/R-mediated membrane translocation and phosphorylation of PKCβ2, but not PKCβ1, PKCδ, or PKCε.21 To explore the protein expression of aPKC subfamily members (PKCζ and PKCλ) in intestinal I/R injury, we evaluated related membrane proteins in intestinal tissues subjected to 45 min of ischemia, followed by 60, 120 or 240 min of reperfusion. Here, PRKCH is linked to ischemia.