Compound 4b emerged as a promising hit molecule as it manifested excellent antineoplastic potency against both tested cell lines (IC50 values of 9 and 12 μM, respectively) beside its ability to inhibit the cell surface expression of CD133 by 50% suggesting a promising potential of effectively controlling the tumour by eradicating the tumour bulk and inhibiting the proliferation of the CSCs. Here, PROM1 is linked to neoplasm.