Accordingly, the ability of the daf-2 mutation to increase lifespan is entirely dependent on DAF-16.12 To determine whether signaling through DAF-16 is required for the beneficial effect we observe in worm models of PD, we used RNAi to knockdown DAF-16 levels in LRRK2(G2019S);daf-2 worms. Here, LRRK2 is linked to Parkinson disease.