To further confirm the role of MUC1 O-glycosylation in MUC1-mediated cell resistance to anoikis, we stably transfected human colon cancer SW620 cells, which express high level of MUC1 (Figure 6a), with shRNA C1GT and control shRNA and generated C1GT stably suppressed cells. This evidence concerns the gene MUC1 and malignant colon neoplasm.