AKT1 and neoplasm: The NCI-60 panel is a well-characterized collection of cell lines representing nine different tumor types.19 Focusing on the 54 cell lines in this panel that were derived from solid tumors, we exposed each cell line to saturating concentrations of 60 different growth factors or cytokines and, following 30 min of stimulation, measured the level of phosphorylated Akt at serine 167 (p-Akt (S473)) by ELISA (Figure 1a, Supplementary Table S1).