To characterize the expression of AXL in vitro, we analyzed the expression of the tyrosine kinase, EMT (CDH1 and VIM), and basal (EGFR, KRT5, and KRT6A) markers in 15 breast cancer cell lines by quantitative real-time PCR (qRT-PCR; Figure 3a). The gene discussed is AXL; the disease is breast carcinoma.