Following this discovery, analyses of adiponectin-deficient mice25–28 as well as adiponectin transgenic mice29,30 provided insights into the long-term effects of adiponectin, where adiponectin-deficient mice were shown to be associated with insulin resistance, impaired glucose tolerance, dyslipidemia, and hypertension, while adiponectin transgenic mice were shown to be associated with improvements in insulin resistance and diabetes mellitus. This evidence concerns the gene ADIPOQ and Impaired glucose tolerance.