[2, 3] We used a commercially available next generation sequencing assay (Machaon Diagnostics, Inc.)to test for mutations in the following twelve genes with known pathogenic mutations associated with atypical HUS: CFH, MCP, CFI, CFB, C3, CFHR1, CFHR3, CFHR4, CFHR5, thrombomodulin, plasminogen, and DGKE. DGKE sequencing included all exons plus 25 bp of flanking intronic DNA, as well as the previously reported c.888 + 40A > G intronic mutation. The gene discussed is CFH; the disease is atypical hemolytic-uremic syndrome.