Examination of aspirin effects on NF-κB signalling using this alternative protocol revealed that prolonged treatment of colorectal cancer cells with pharmacologically relevant doses (0.5–5 mM) of aspirin alone actually stimulates the NF-κB pathway, as evidenced by phosphorylation/degradation of IκB and nuclear translocation of RelA [8] (Figure 1). The gene discussed is NFKB1; the disease is colorectal cancer.