To address this issue and determine a possible genetic association of the rs33912345:c.421A>C and rs10483727 (SIX1‐SIX6) SNPs, we sequenced the SIX6 locus in a cohort of south Indian POAG patients and age‐matched controls and further compared the resulting genotypes with the RNFL and VCDR parameters. Here, SIX1 is linked to open-angle glaucoma.