Here we performed ChIP-PCR, with primers (spanning from +40 to −230 bp with respect to the hTERT transcription start site) flanking the putative NME2-binding site, first in HT1080 fibrosarcoma cells and then in HCT116 colon cancer cells to confirm NME2 occupancy at the hTERT promoter (Fig. 1a and supplemental Fig. S1a). This evidence concerns the gene NME2 and malignant colon neoplasm.