NME2 and colonic neoplasm: Here we performed ChIP-PCR, with primers (spanning from +40 to −230 bp with respect to the hTERT transcription start site) flanking the putative NME2-binding site, first in HT1080 fibrosarcoma cells and then in HCT116 colon cancer cells to confirm NME2 occupancy at the hTERT promoter (Fig. 1a and supplemental Fig. S1a).