To confirm the role of Tid1-S in the mitochondrial localization of EGFR, we examined the effect of Tid1-S overexpression on the mitochondrial localization of EGFR in a human lung cancer cell line that expresses a low level of endogenous Tid1 (CL1-5 cells).17 Overexpression of HA-tagged wild-type Tid1-S (Tid1-S-Wt) (Figure 2a) greatly enhanced the co-localization of EGFR with Tid1-S in mitochondria (Figure 2b). This evidence concerns the gene EGFR and lung cancer.