TNF and infection: To establish if SH-mediated inhibition of phospho-p65 expression resulted in decreased production of NF-κB-dependent cytokines, RAW 264.7 cells were infected with wt rBRSV or rBRSVΔSH in the presence or absence of TNF-α and cytokine expression in culture supernatants was analysed by multiplex ELISA, 24 h post infection.