IFNG and tuberculosis: In this research, a cELISA based on mAb 3B8 was developed, the analyses of clinic serum samples from cattles with bovine TB and healthy cattles demonstrated that the diagnostic specificity and sensitivity were 54.2% (26/48) and 83.5% (167/200), respectively, and the total coincidence of the cELISA method with skin test and IFN-γ assay was 74.5 and 71.8%, respectively.