Another recently published study described the application of exogenous seeding material, either the insoluble fraction of TDP-43 derived from HEK293 cells transfected with WT FLAG-tagged TDP-43 (WT FL TDP-43) or ALS patient tissue, via chemical transfection methods to acceptor HEK293 cells over-expressing WT FL TDP-43 [27]. The gene discussed is TARDBP; the disease is amyotrophic lateral sclerosis.