IRS1 and hepatocellular carcinoma: To elucidate the roles of Irs1 and Irs2 in HCC development, LIrs1KO, LIrs2KO mice and the respective control (Irs1lox/lox and Irs2lox/lox) mice were injected with 25 mg/kg of DEN at the age of 15 days.