Over increasing durations of hyperglycemia exposure, SP1 mRNA levels were evaluated in HRECs (Figure 4(a)) and ARPE-19 cells (Figure 4(b)) after normalization relative to the mRNA levels of Pumilio homolog 1 (PUM1) and peptidylprolyl isomerase A (PPIA), respectively. This evidence concerns the gene PUM1 and Hyperglycemia.