By comparing the percentages of T cell subpopulations isolated from tumor-bearing mice in the different experimental groups, we observed by flow cytometry that more CD4+ and CD8+ T cells infiltrated into the tumor site in the LM4Δhly::E7 and LM4Δhly::esat-6 groups compared with the PBS group (Figures 6A,B). This evidence concerns the gene CD8A and neoplasm.