They were identified in different cancer types, including CRC, and predicted to alter the target sites for MIR4321, MIR4662a, MIR181b, and MIR10a. The authors of these studies were not directly searching for miRNA target site mutations, but the frequency of these findings support the significance of describing the functional impact of a new somatic mutation in E2F1. This evidence concerns the gene E2F1 and cancer.