E2F1 and melanoma: Our study used reporter assays and showed that after MIR205-5p overexpression, mutated E2F1 (at the miRNA target site) has a 50% higher luciferase activity when compared to WT E2F1. Regulation of E2F1 by MIR205 has already been validated for melanoma, where overexpression of MIR205 directly inhibits E2F1 expression and prevents tumor progression in vivo [25].