To test this hypothesis, here we used a rat model of RM-AKI produced by the injection of glycerol to the hind paw and examined the kinetics of gene expression of Bach1, HO-1, and ALAS1 (the rate-limiting enzyme in heme biosynthesis), and Bach1 protein expression in the cytosolic and nuclear fractions of kidney cells after glycerol treatment. Here, ALAS1 is linked to acute kidney injury.