In contrast, the EC50 value of GrB(R201K)-TTC against REH cells was 5 nM (Fig 6B), which is in the same range or slightly lower than other granzyme B fusion proteins targeting cancer cells such as GbR201K-Ki4(scFv) against L540cy cells (EC50 = 1.7 nM), GbR201K-scFv1711 against RD cells (EC50 = 21.1 nM), or the anti-malarial activity of granzyme B (EC50 = 176 nM) [30, 32, 54, 56, 78]. The gene discussed is GZMB; the disease is cancer.