UBE2K and leptospirosis: Purified, recombinant LigA7’-13 was stable in solution, however, precipitation was observed in purified LigB0-7 when kept for prolonged periods at 4°C. We evaluated the antigenicity of the purified Lig proteins by immunoblot analysis and demonstrated that both LigA7’-13 and LigB0-7 were recognized by sera from hamsters infected intraperitoneally with L. interrogans, and by sera of leptospirosis patients from Brazil in convalescent phase (Fig 1).