In the present study, we use these ERα- and ERβ-expressing PRL-HeLa, the widely utilized ER-positive MCF-7 breast cancer, and the AR-positive LNCaP prostate cancer cell lines in high content/high throughput assays to characterize and classify seven, less well studied, potential BPA substitutes identified by Valspar during an internal yeast-based in vitro assay (data not published). The gene discussed is ESR1; the disease is Familial prostate cancer.