Based on the observations that ATG5 and ATG7 knockdown reduce the expansion of AML CD34+ cells in vitro, we determined whether this would also occur in vivo. To exclude the possibility that the knockdown of ATG5 or ATG7 affected cell migration, in vitro transwell experiments were performed with the OCIM3 and MLOM13 cell line. Here, ATG5 is linked to acute myeloid leukemia.