The results of the current study demonstrated that treatment of the A375.S2 melanoma cells with cudraflavone C induced increased expression of members of the pro-apoptotic Bcl-2 family (Puma, Bax, Bad, Bid), Apaf-1, and cytochrome c, which could be suppressed by pre-treating the cells with MitoTEMPOL. The gene discussed is APAF1; the disease is melanoma.