In Tlx3cko mice, although histamine-dependent acute itch was markedly attenuated, a TRPV1 antagonist still alleviated the chronic itch evoked by dry skin or ACD, suggesting that TRPV1 was required for mediating chronic itch induced by other endogenous pruritogens with distinct mechanisms. Here, TRPV1 is linked to granular corneal dystrophy type II.