To examine TSPAN1 protein expression in clinical prostate cancer tissue we further showed that staining by this antibody was blocked by pre-incubation with a blocking peptide (Supplementary Figure 2C) and by detection of siRNA mediated protein depletion by immunohistochemistry in Formalin Fixed Paraffin Embedded (FFPE) cell pellets (Supplementary Figure 2D). This evidence concerns the gene TSPAN1 and prostate carcinoma.