To investigate how F991 reduced DSS-induced colitis, we used ELISA to analyze the levels of proinflammatory cytokines, including IL-6 and TNF-α, and the levels of the anti-inflammatory cytokine IL-10 in cultured supernatant from DSS-colitis tissues after F991 treatment on the 2nd, 4th, 6th days. This evidence concerns the gene IL6 and colitis.