However, this frequency is rather low in this pediatric cohort (CD8+CD4-FOXP3+/(CD8+CD4-FOXP3++CD4+CD8-FOXP3+ = 3.7%) and similar low frequencies of CD8+CD4-FOXP3+ were found in the adult cohorts, in which we could perform the epigenetic validation with the TSDR analysis (2.8% for AIH and 3.8% for acute cellular rejection) [13, 17]. This evidence concerns the gene FOXP3 and autoimmune hepatitis.