Similar to the in vitro results at 6 dpi (S1 Fig), the rHEP5.0-GML-mRFP-infected CA1 cells also showed a significantly higher intensity of anti-N antibody labeling than rHEP5.0-GctL-mRFP- and rHEP5.0-CVSG-mRFP-injected rats (p < 0.0001, one-way ANOVA; post-hoc t-test, Bonferroni-corrected, p < 0.00033 for rHEP5.0-CVSG-mRFP infection vs rHEP5.0-GML-mRFP infection and rHEP5.0-GctL-mRFP infection vs rHEP5.0-GML-mRFP infection, Fig 2E, 2H and 2K, and S3 Fig). This evidence concerns the gene GML and infection.