Interestingly, when we analyzed the TS3 spheroids that were generated from MDA‐MB‐231 cells by qPCR, we observed a significant upregulation in LKB1 expression in the tumor spheres compared to the cells grown as monolayers (P = 0.013) and to TS1 (P = 0.002) (Fig. 7). This evidence concerns the gene STK11 and neoplasm.