The inhibition of endogenous GTSE1 dramatically decreased the number of cancer cells during migration (QGY-7703: 122.38 ± 18.53 vs. 42.25 ± 7.15, P < 0.001; SMMC-7721: 252.25 ± 21.18 vs. 166.88 ± 39.15, P < 0.001; Fig. 3a) or invasion (QGY-7703: 90.50 ± 19.93 vs. 58.13 ± 14.86, P = 0.002; SMMC-7721: 66.63 ± 20.18 vs. 34.12 ± 4.26, P = 0.002; Fig. 3b) through transwell membranes. Here, GTSE1 is linked to cancer.