To investigate a putative reverse signaling of transmembrane CXCL16 upon binding of its known receptor CXCR6, for the first approach, we used different CXCL16-positive, CXCR6-negative glioblastoma cell lines and stimulated them with 25 ng/mL recombinant CXCR6 for 10 or 15 min. This evidence concerns the gene CXCL16 and glioblastoma.