Furthermore, we stimulated LOX melanoma cells stably expressing a CXCL16 variant that lacks the cytoplasmic tail due to truncation (LOX-ΔCXCL16) with recombinant and membrane expressed CXCR6, as well as control membrane fractions and did not observe any ERK1/2 phosphorylation either (Figure 3C). This evidence concerns the gene MAPK3 and melanoma.