In the forelimbs of WT mice, cells immunoreactive for Gal-3 were identified in larger vessels with staining seen in some skeletal muscle nuclei (Figure 5A, left), whereas in the forelimb tumor of Tsc2cKOPrrx1-cre mice, areas of dense Gal-3 positivity were observed in both vascular and perivascular cells (Figure 5A, right). This evidence concerns the gene LGALS3 and neoplasm.