To confirm the role played by LTB4 in ER stress, oxidative stress, and insulin resistance, 5-LO was knocked down using 5-LO siRNA to 30% of the control level (Fig. 3a), and LTB4 production by PA was also markedly inhibited by 5-LO siRNA (Fig. 3b). This evidence concerns the gene ALOX5 and Insulin resistance.