Although our results suggest that local LTB4 elicits direct effects on myotubes, the possibility that LTB4 produced locally in skeletal muscle induces macrophage infiltration into skeletal muscle in vivo, and thus, leads to inflammation and insulin resistance cannot be excluded, since in a previous study, macrophage depletion and BLT1 inhibition improved glucose intolerance in an additive manner19. Here, LTB4R is linked to Insulin resistance.