In our study, the phosphorylation level of HSP27, Chk1, p53, and p38, as well as cleavage of caspase-7 was elevated in ANKRD49-silenced glioma cells, adding the evidence that depletion of ANKRD49 could suppress malignant glioma cell growth via the induction of cell-cycle arrest and apoptosis. This evidence concerns the gene ANKRD49 and central nervous system cancer.