group 1 included culture confirmed positive sera of brucellosis (n = 15) out of 15 samples 14 was found to be positive by rOmp2a porin protein as antigen and 1 as negative (Fig. 6), in group 2 out of 54 sera samples positive by both RBPT (n = 28) and STAT (n = 26), in RBPT (n = 28), 23 samples were picked up as positive by rOmp2a iELISA and 5 samples were found to be negative by iELISA. This evidence concerns the gene VDAC1 and brucellosis.