Moreover, we observed that Gpr43 mRNA expression slightly increased in the mesenteric adipose tissue SVF with progression of obesity in vivo and was also higher in LPS-stimulated Raw264.7 cells (mouse macrophage cell line) than in non-treated Raw264.7 cells in vitro (S1D and S3E Figs). The gene discussed is FFAR2; the disease is obesity due to melanocortin 4 receptor deficiency.