G6PD and colorectal carcinoma: Non-viral vectors for gene delivery have become a rapid-developing field recently.22 We have established a new gene delivery system based on cationic poly(ω-pentadecalactone-co-N-methyldiethyleneamine-co-sebacate) (PPMS) polyplex with high gene transfection capability.23, 24 The modified G6PD shRNA#1/PPMS polyplexes were prepared according to our previous study.31 First, the knockdown efficiency was evaluated in CRC cells using PPMS polyplexes loaded with G6PD shRNA#1.