G6PD and colorectal carcinoma: When ROS accumulation overwhelms the cell detoxification capacity, ROS cytotoxicity damages the mitochondria and cell membranes, causing cell death.1 We further showed that cellular apoptosis and necrosis were also increased in G6PD-knockdown CRC cells treated with H2O2 (200 μM) for 48 h detected by the Annexin-V/PI assay (Figures 2f and g).