ERBB2 and breast cancer: The luminal A (LumA), luminal B (LumB), HER2-enriched (HER2-E), basal-like, and normal-like breast cancer subtypes were initially defined through unsupervised clustering analysis of global gene expression from RNA extracted from frozen tissue.1 A 50-gene qPCR assay (PAM50) was developed to identify the intrinsic biological subtypes using RNA isolated from more readily available formalin-fixed, paraffin-embedded (FFPE) tissue.