The MFI of FoxP3 in Treg and Tfr fractions, meanwhile, was not significantly different between the CIS and HC groups (P⩾0.69 for all, data not shown) and was not significantly correlated with the percentage of IL-10+ CD4+ T cells (P⩾0.35 for all, data not shown). This evidence concerns the gene CD4 and in situ carcinoma.